可动苯基杆菌对LAS生物降解机理的初步探讨

Preliminary Study on the Mechanism of LAS Biodegradation With Phenylobaterium Mobile

从不同地区直链烷基苯磺酸钠(linear sodium alkylbenzenesulfonate,简称LAS)富集地带取回的污水样中分离纯化得到一株能高效降解直链烷基苯磺酸钠的菌株——可动苯基杆菌(phenylobaterium mobil GZ6)。该菌细胞为短杆状,大小为(0.5～0.8)μm×(1.0～2.0)μm,其生长pH为6.0～10.0,最适生长pH为7.0,生长温度为4～40℃,最适生长温度为28℃。本实验用温度为28℃,转速为170r/min的振荡摇床培养GZ6菌,并实时监测其对LAS的降解情况。由紫外分析图发现,在GZ6对LAS降解的第8h,出现吸光度变化的最大值点,取第8h的样品用HPLC法分离,并用MS法进一步佐证紫外吸收的分析结果,探讨了GZ6菌对LAS的降解机理。

In different districts with plenty of LAS, a Phenylobaterium mobile GZ6 which can efficiently degrade linear alkylbenzenesulfonate (LAS) is selected by separating and purifying . It is aerobic gram-negative short-rod (0.5 to 0.8 by 1.0 to 2.0 urn). Optimum growth occurs at 28 ℃ and pH = 7.0. GZ6 is cultivated by the shaking table at 28 ℃ and 170 r/min. Through ultraviolet (UV) spectrum analysis, the authors find that at the 8th hour in the process of the degradation of LAS by GZ6, there occurs the biggest ABS. So through separating the 8th hour s sample by HPLC and further analyzing by MS, they give out the primary mechanism of degradation of LAS by GZ6.