聚天青A/辣根过氧化物酶电极的制备及性能

Preparation and Properties of Poly(azure A)/Horseradish Peroxidase Enzyme Electrode

以循环伏安法在玻碳电极表面电化学聚合天青 A的同时实现了辣根过氧化物酶 ( HRP)的固定化 ,制备了聚天青 A/辣根过氧化物酶 ( PAA/HRP)电极。该电极对 H2 O2有很好的生物电催化还原作用 ,可在没有电子传递体的情况下催化 H2 O2 的还原 ,催化电流与 H2 O2 浓度在 1 .0× 1 0 - 6～ 6.0× 1 0 - 3mol· L- 1的范围内呈良好的线性关系 ,相关系数 r=0 .9993( n=1 6)。该酶电极制备简单 ,重现性良好 ,用于 H2 O2 的测定 ,可使用 60次以上。

With the electropolymerization of azure A by cyclic voltammetry, horseradish peroxidase (HRP) was immobilized in the poly(azure A) membrane to construct poly(azure A)/horseradish peroxidase (PAA/HRP) enzyme electrode. The obtained enzyme electrode exhibited higher catalytic activity for the reduction of H 2O 2 in the absence of electron transfer media, and the linear current response to H 2O 2 was in the range of 1.0 × 10 -6 to 6.0×10 -3 mol·L -1 with the correlation coefficiency r =0.999 3 ( n =16).This PAA/HRP enzyme electrode can be easily fabricated and has the perfect reproducibility. It can be used for more than 60 times for detecting H 2O 2 solutions.