花生不同部位对提取DNA的影响

Extraction of DNA from Different Parts of Peanut

采用CTAB法和SDS法,分别从花生的根尖、下胚轴、种子、幼叶中提取基因组DNA,所提DNA片段长度都在21kb以上;同种方法中部位之间的DNA纯度没有差异,产率差异极其显著,从种子、根尖、下胚轴到幼叶产率依次升高,CTAB法中产率从128.5～498.5ng/mg,SDS法中产率从225.0～542.6ng/mg;两种方法中,相同部位之间只有叶片在纯度上差异显著,产率上相同部位之间的差异极其显著。用CTAB法从叶片中提取DNA,采用RAPD方法,对19个花生品种的基因组进行扩增,结果显示所提DNA满足分子分析的要求。RAPD可用于亲本和品种的鉴定。

DNA was extracted from seeds, root tips, hypocotyls and leaflets of peanut using the CTAB method and SDS method, respectively. The results showed that the MW of the DNA samples was higher than 21 kb,and that using the same method, the purity of the DNAs extracted from different parts was not different, but the DNA production rate significantly differed, with leaflets producing the largest amount and seeds producing the smallest amount. The DNA production rate varied from 128.5 to 498.5 ng/mg with the CTAB method, and ranged from 225.0 to 542.6 ng/mg with the SDS method. Between the extraction methods, the DNA production rate of the same tissue type significantly differed, and as far as the DNA purity of the same tissue type was concerned, only leaflets significantly differed. RAPD analysis among 19 peanut cultivars using DNAs extracted from leaflets with the CTAB method indicated that DNAs were up to the standard for molecular analysis and RAPD could be used to identify the cultivars of peanut.