Ⅰ型单纯疱疹病毒糖蛋白G基因片段的克隆表达及初步鉴定

Cloning,Expression of HSV-1 gG Gene Fragment and Identification of the Expressed Protein

通过计算机分析Ⅰ型单纯疱疹病毒糖蛋白G的氨基酸序列 ,筛选出HSV1 gG蛋白中优势抗原决定簇位点 ,用PCR技术扩增克隆含强抗原决定簇较集中的基因片段。将该段基因克隆至质粒表达载体pGEX 4T 2内 ,转化大肠杆菌TG1 ,构建成功了高效表达Ⅰ型单纯疱疹病毒糖蛋白G基因的工程菌。用纯化的表达蛋白HSV1 gG GST作抗原ELISA分析证实有较好的抗原性和特异性 。

The herpes simplex virus type 1 glycoprotein G gene fragment containing dominant antigenic determinants confirmed by analysing amino acid sequences was cloned by PCR and inserted into plasmid vector pGEX 4T 2,then the recombinant plasmid was transformed to E.coli TG1.The engineering E.coli expressing HSV1 gG/GST was established.The purified expressed protein showed good antigenicity and specificity by ELISA test.The study is of great significance in early diagnosis of HSV.\