利用固定化细胞发酵生产2-酮基-D-葡萄糖酸的研究

Immobilization of Pseudomonzs Cell for Production of 2-keto-D-gluconic Acid

用海藻酸钠、PVA及PVA与海藻酸钠以一定比例混合,制成2-KG产生菌的固定化细胞,经发酵实验表明,5％海藻酸钠固定化细胞的产酸能力及稳定性都优于其它固定化细胞。这种固定化细胞可以连续发酵6次以上,在4℃条件下,储存30天,仍具有很好的活性,与储存前产酸能力相同。

The method for cell immobilization was tested by Na-alginate and PVA. The experiment results shown that the immobilized cells by 5 % Na-alginate was for higher yield and stability than the other immobilized cells. The immobilized cells were used over 6 batches of repeated use without noticeable breakage .