摘要
采用比较试验的方法 ,为树莓 (RubusidaeusL .)快速繁殖筛选了诱导、增殖和生根培养基。建立了树莓的再生系统。以MS为基本培养基 ,附加 0 .75～ 1 .5 0mol·L-1 BA +0 .1 0mol·L-1 IBA诱导芽的萌动 ;附加 0 .5 0～1 .0 0mol·L-1 BA +0 .2 5mol·L-1 GA用于增殖 ,产生丛生芽 ;培养基 1 / 2MS +0 .5 0～ 1 .0 0mol·L-1 IBA用于根的诱导。同时探索了抑制树莓组培中苗木体内细菌的途径 ,找到了较好的抗生素配比 。
We used comparative experimentation and selected the induced, increased and rooted medium for the rapid propagation of Rubus ideaus L., Then we established a plant regeneration system of Rubus ideaus L. MS+0.75～1.50BA mol·L -1 +0.1 mol·L -1 IBA was suitable for inducing shoots; MS+0.5～1.0 mol·L -1 BA+0.25 mol·L -1 GA was good for induction of bud; 1/2 MS+0.5～1.0 mol·L -1 IBAwas good for induction of roots. At the same time we studied the way to inhibiting bacteria in the body of Rubus ideaus L. during the tissue culture and found a better ratio of antibiotic,which established foundation for the use of rapid propagation in productive practice.
出处
《新疆农业大学学报》
CAS
2003年第1期28-30,共3页
Journal of Xinjiang Agricultural University
基金
新疆林业局资助项目
关键词
树莓
植物组织培养
快速繁殖
抑菌
Rubus ideaus L.
plants tissue culture
rapid propagation
inhibiting bacteria
