紫草试管快速繁殖

The Formation of in Vitro Shoot of Lithospermum Erythrorhizon

紫草(LithosPermum erythrorhizon)进行组织培养形成试管苗,应用LS基本培养基。用试管苗茎尖作外植体诱导愈伤组织即,使培养基的激素量减少到10％,愈伤组织诱导率仍比原植物作外植体高50％。当培养基添加05mg/l激动素和2mg/16苄基嘌呤,试管苗迅速增殖。当培养基无机盐减少一半,添加1mg/l的激素Ⅰ,接种物低温处理一定天数,根诱导率达80％至90％。

The tissue culture of Lithospermum erythrorhizon was carried out to form in vitro shoot. The LS basal medinm was employed. Even if the hormon of the medium was decreased to 10%, the callus inducing rate of in vitro shoot, which acted as explant, had increased by50% over that of the adult inact plant. When medium wassupplemented with 0.5mg/l kinetin and 2mg/l benzyladenme, the vitro shoot quickly multiplied. When the mineral salt amount of the medium was half those of basal medium and also supplemented with 1 mg/l Hormone Ⅰ and the moculum had been dealt with Low temperature for certain days, the root inducing rate was from 80%to90%.