DREB1A基因植物表达载体的构建

Construction of plant transformation vector of DREB1A gene

以 p Bch质粒为基础 ,构建了分别由 35 s启动子、 rd2 9A启动子调控的 DREB1A基因双子叶植物表达载体p BD35 s、p BD2 9A,其植物选择标记为新霉素磷酸转移酶基因 (N PT ) ,适用于双子叶植物的遗传转化。以 p CU质粒为基础 ,构建了分别由 E12启动子、 rd2 9A启动子调控的 DREB1A基因单子叶植物表达载体 p CDE12、p CD2 9A,其植物选择标记为乙酰 Co A转移酶基因 (bar) ,适用于单子叶植物的遗传转化。并分别通过三亲本杂交法和冻融法将重组质粒导入根癌农杆菌 L BA4 4 0 4中 ,为通过农杆菌介导法将

Four plant transformation vector were constructed in this experiment.Plasmid pBD35s and pBD29A,which derived from pBch,including DREB 1A gene controlled by CaMV35s promoter and rd29A promoter respectively.Plasmid pCDE12 and pCD29A were derived from pCU,in which the DREB1A gene were regulated by E12 promoter and rd29A promoter respectively.Then the recombination plasmids were introduced into agrobacterium LBA4404 by tri parental conjugation and direct entering ways.$$$$