摘要
在MS培养基上 ,附加 5mg/L病毒唑培养唐菖蒲 ,再经 38～ 40℃热处理 ,切取微茎尖两次 ,去除了危害唐菖蒲的 3种主要病毒TMV、CMV和TVY。以MS +BA 0 5mg/L +NAA 0 5mg/L +KT 1mg/L为继代培养基 ,1/ 2MS无激素培养基为成球培养基 ,进行快繁获得了脱毒种球 ,移植后 ,植株生长发育状况得到明显改善。
A study on tissue culture of gladiolus hybridus of'Chenghongjiao'and'Ziyinghua'was carried out in MS medium with different phyto-hormone BA,KT and NAA.A MS medium supplemented with BA 0.5 mg/L+NAA 0.5 mg/L+KT 1 mg/L was for the regenerated and 1/2MS medium without hormone was for bulbs forming.The three main viruses of TMV,CMV and TVY in gladiolus were removed from cultural plant of gladiolus in the regenerated medium through 5-20 mg/L concentration of ribavirin,treatment of high temperature 38-40℃ and 2 times of meristem-tip culture.
出处
《园艺学报》
CAS
CSCD
北大核心
2003年第3期358-360,共3页
Acta Horticulturae Sinica
