血管内皮生长因子异常表达对肺癌诊断和预后价值

Clinical value of abnormal expression of vascular endothelial growth factor on diagnosis and prognosis of patients with lung cancer.

目的:探讨血管内皮生长因子(VEGF)在肺癌组织中的表达及其临床价值以及VEGF与肿瘤生长、转移及预后的关系。方法:对38例肺癌组织以免疫组织化学法研究VEGF在肿瘤组织的胞内分布及其表达;同时以ELISA法测定临床上肺癌、良性肺病患者及正常对照血清中的VEGF水平。结果:肺癌组织中VEGF位于胞质和胞膜,呈棕黄色颗粒状过度表达,Ⅲ期、Ⅳ期肺癌和伴远处转移者阳性率为90%,肺癌患者血清中VEGF水平显著高于正常对照(P<0.01),但在良恶性肺病组间未见明显差异,经抗感染药物治疗后,良性肺病患者血清VEGF水平明显下降。结论:肺癌发生和发展过程中VEGF呈过度表达,检测患者血清VEGF可以作为肺癌诊断和预后的有用指标。

Objective:To explore changes and clinical values of vascular endothelial growth factor (VEGF) in diagnosis and prognosis of patients with lung cancers. Methods:The VEGF cellular distributions and expression in 38 specimens of patients with lung cancer were investigated with immunohistochemistry assay. The levels of VEGF in sera of patients with lung cancer, benign lung diseases and healthy controls were detected through ELISA method. Results:The VEGF positive stain was 76% in 38 cases of lung cancer specimens. The 89% rate of VEGF stain was found for clinical stage III cases and 92% for stage IV lung cancers. The significantly higher expression of VEGF was evidenced in patients with lymph node metastasis (84%), distant metastasis (90%), and lung cancers with lower histological differentiation (89%), respectively. The level of serum VEGF in the patients with lung cancer was significantly higher than that in the healthy control group(P<0 01) No significant difference of serum VEGF levels was detected between cases with lung cancer and patients with benign diseases(mainly inflammatory disease) . However, the VEGF level of cases with benign diseases was decreased significantly after patients with anti-inflammation medication. Conclusion:The present data suggest that the expression of VEGF has a significant correlation among growth, invasion and lymphonode metastasis. VEGF may be an independent predictor of prognosis .The level of serum VEGF in patients with lung cancer may be a useful indicator for tumor angiogenesis and metastasis.