表皮细胞生长因子及其受体在胎儿和成人肠道组织中的表达特征

EXPRESSIVE CHARACTERISTICS OF EPIDERMAL GROWTH FACTOR AND ITS RECEPTOR IN TISSUES OF FETAL AND ADULT INTESTINES

目的 探讨表皮细胞生长因子 (EGF)及其受体 (EGFR)在不同发育阶段人小肠组织中的表达特征及其可能的生物学意义。 方法 2 4例被测标本中包括胎儿 (胎龄 13～ 31周 )的小肠组织 18例 ,成人 (16～ 5 4岁 )的小肠组织 6例。用病理学技术和免疫组织化学方法确定 EGF及 EGFR两种蛋白在胎儿、成人小肠组织中的定位以及表达量的变化规律。 结果 EGF及 EGFR在不同胎龄的胎儿和成人小肠组织中均有阳性表达。随着胎儿生长发育 ,小肠组织中 EGF和 EGFR的蛋白含量逐渐增加 ,这一变化趋势一直保持到成人小肠组织中。其中 EGF主要存在于小肠黏膜上皮细胞、黏膜下层的血管内皮细胞内和浆膜上皮细胞的胞浆和胞外基质中 ,EGFR则分布于这些细胞的细胞膜上。 结论EGF及 EGFR在不同发育阶段的小肠组织中呈阳性表达 ,这种细胞因子可能以自分泌或旁分泌方式调节人肠道的生长发育、结构和功能的维持 。

Objective To explore the expressive characteristics of epidermal growth factor (EGF) and its receptor (EGFR) in tissues of fetal and adult intestines. Methods The expression intensity and distribution of EGF and EGFR were detected with pathological and immunohistochemical methods in 6 specimens of adult (16 54 years) intestines and 18 specimens of fetal intestines with different gestational ages (13 31 weeks). Results Positive protein particles of EGF and EGFR could be detected in tissues of fetal and adult intestines. The protein expressions of EGF and EGFR were elevated progressively with the gestational age. EGF was mainly located in the cytoplasm and extracellular matrix of intestinal villus cells, endothelial cells and tunica serosa epithelial cells, while EGFR chiefly distributed in the cellular membrane of these cells. Conclusion The endogenous EGF and EGFR might be involved in the intestinal development at embryonic stage, in the structural and functional maintenance at adult stage, and in the wound healing after injury.