丹参酮Ⅱ_A对四氯化碳损伤原代培养大鼠肝细胞的影响

Protective Effects of Tanshinone Ⅱ_A on Injuried Primary Cultured Rat Hepatocytes Induced by CCl_4

目的 :研究丹参酮ⅡA 对四氯化碳 (CCl4)损伤原代培养大鼠肝细胞的保护作用。方法 :原位灌流分离大鼠肝细胞培养 36h后加入丹参酮ⅡA,同时造成CCl4损伤 ,于损伤 3h测培养液中丙二醛 (MDA)、一氧化氮 (NO)的含量 ,谷丙转氨酶 (ALT)、乳酸脱氢酶 (LDH)、超氧化物歧化酶 (SOD)的活性 ,并用MTT法测肝细胞存活率。结果 :丹参酮ⅡA 明显改善细胞存活率 ,抑制CCl4引起的ALT、LDH活力的升高 ,同时抑制MDA、NO的产生及提高SOD活力。结论 :丹参酮ⅡA 能有效抑制CCl4造成的原代培养大鼠肝细胞损伤。

Objective: To investigate the effects of tanshinone Ⅱ A on carbon tetrachloridlde(CCl 4) intoxicated primary cultured rat hepatocytes Methods: The rat hepatocytes were separated by perfusion with collagenase solution Tanshinone Ⅱ A was added to the cultured medium after incubating the rat hepatocytes for 36 hours At the same time, CCl 4 was added to induce hepatocytes injury for 1 h The levels of ALT, LDH, NO, SOD, MDA and cell viability after cultivation for 3 h were determined respectively Result: Tanshinone Ⅱ A significantly improved cell viability, inhibited the decrease of SOD activity and the increase of ALT, LDH, MDA, NO levels Conclusion: Tanshinone Ⅱ A protected effectively the primary cultured rat hepatocytes against CCl 4 induced injury