迪卡配套系猪RYR1的PCR分析及其利用的研究

Diagnosis and Application of DIKA kit Department Pigs Genotyoes for Halthane at DNA Level by PCR Amplification

试验从鸡东县永安猪扬随机抽取迪卡配套系猪B系和E系 4 2头 ,通过PCR扩增技术和酶切鉴定 ,采用克隆技术进行测序 ,来检测是否含有氟烷基因序列。经过以上技术检测这 4 2头猪均没有氟烷基因。

This experiment to select by examinations with the machine from Yong an PIG factory Jidong county permanent stability pig field DIKA kit department pig B department with the department of E,pass the PCR expands the technique adds the enzyme slice to authenticate to examine these 42 pigs to all have no should arouse to synthesize to advertise for,then pass clone technique proceed to measure the preface.This technique to the development of the pig-raising industry contain important and realistic meaning.