肝靶向葫芦素B聚乳酸-羟基乙酸毫微球的小鼠体内分布研究

Study on the distribution of the liver targeted cucurbitacin B loaded PLGA nanospheres in mice

目的研究葫芦素B聚乳酸-羟基乙酸毫微球小鼠体内分布。方法小鼠尾静脉给药,HPLC法测定葫芦素B在小鼠血浆、心、肝、脾、肺、肾、脑中的浓度。结果葫芦素B聚乳酸-羟基乙酸毫微球主要分布于肝,给药15 min,肝中聚集达最大,为给药剂量的47.15%。结论葫芦素B聚乳酸-羟基乙酸毫微球可实现肝脏被动靶向,所建立的HPLC法用于葫芦素B的体内分布研究,结果良好。

Objective To study the distribution of cucurbitacin B loaded PLGA nanospheres in mice.Methods After admistration to mice by iv,HPLC was used to determine the concentration of cucurbitacin B of plasma,heart,liver,spleen,lung,kidney and brain respectively.Results The distribution of cucurbitacin B in liver was significantly higher than that in any other organs.At 15 min,the uptake of liver reached a maximum,about 47.15% of the dose.Conclusion Cucurbitacin B loaded PLGA nanospheres show passive targeting to liver.The established HPLC method can be used for cucurbitacin B biodistribution study.