反相高效液相色谱法测定齐多夫定棕榈酸酯纳米乳剂药物含量

Determination of zidovudine palmitate in nanoemulsions by RP-HPLC

目的建立测定齐多夫定棕榈酸酯纳米乳剂中药物含量的方法。方法采用Betasil ODS柱(4.6 mm×200 mm,5μm);流动相为甲醇-水(体积比为95:5),流速为1.0 mL·min-1,检测波长为266 nm,柱温为室温。结果在本试验条件下齐多夫定棕榈酸酯与辅料、溶剂及有关物质分离度均符合要求,在40~400 mg·L-1内呈良好的线性关系(r=0.999 9,n=7),齐多夫定棕榈酸酯平均回收率为99.84%,RSD为0.35%(n=3)。结论本方法可用于齐多夫定棕榈酸酯纳米乳剂含量测定。

Objective To establish a RP-HPLC method for the determination of zidovudine palmitate in nanoemulsions. Methods The separation was performed with a Betasil ODS column(4.6 mm×200 mm, 5 μm) at room temperature, and the mobile phase was consisted of methanol-water(95:5), the flow rate was 1 mL·min-1. Zidovudine palmitate was detected at 266nm. Results There was a good linear relationship between A and concentration over the range of 40~400 mg·L-1(r=0.999 9, n=7). The average recovery was 99.84%. The RSD was 0.35%. Conclusion This method is applicable for the determination of zidovudine palmitate in nanoemulsions.