微生物来源抗铜绿假单孢菌化合物X412的分离与鉴定

Isolation and identification of X412 as anti-Pseudomonas aeruginosa compound from microorganism

目的 :对真菌 4 12的代谢产物进行活性跟踪研究 ,分离出具有抗耐药绿脓杆菌活性的小分子化合物X4 12。方法 :采用大孔吸附、溶媒萃取、硅胶柱色谱、重结晶进行分离和纯化 ,以纸片法进行活性跟踪 ,通过UV ,IR ,FABMS ,NMR和元素分析进行化合物的结构鉴定。结果 :分离得到的化合物X4 12的相对分子质量为 4 4 4 ,波谱解析和文献检索初步推定其为麦角甾醇类新化合物。结论 :通过抗耐药绿脓杆菌筛选模型筛选出活性物质X4 12 ,且X4 12为新化合物。

Objective:To isolate X412 from metabolites of 412,a sp.of fungi.Methods:X412 was isolated by X5 resin adsorption,solvent extraction,silica gel column chromatographic separation,and purified by recrystallization.Its activity was tested.Its chemical structure was elucidated by UV,IR,FABMS,NMR and element analysis.Results:X412 has a molecular weight of 444.It is identified as an unknown compound with the structure of ergosterol.Conclusion:X412 is recognized as a first reported new compound with anti Pseudomonas aeruginosa activity.