狼疮性肾炎患者血清和尿中IL-6检测及其临床意义

Interleukin-6 measurement in serum and urine in patients with lupus nephritis and its clinical significance

目的 :探讨细胞因子在狼疮性肾炎发病机制中的作用。方法 :应用白细胞介素 6(IL 6)依赖细胞株KD83 ,检测狼疮性肾炎 (LN)患者活动期组、稳定期组和正常对照组血清和尿中IL 6的活性。结果 :活动期组LN患者血清和尿中IL 6活性均明显高于稳定期组和正常对照组 (P <0 0 1) ,而稳定期组LN患者和正常对照组之间无显著差异 (P >0 0 5 ) ;活动期组LN患者血清和尿中IL 6活性均与血沉 (ESR)呈正相关 (r =0 683 0和r =0 5 767,P <0 0 5 ) ,而与血清补体C3、抗核抗体(ANA)、抗ds DNA抗体均无相关关系 ;活动期组LN患者血清IL 6活性与血清IgG呈正相关 (r=0 83 5 0 ,P <0 0 1) ,与补体C4呈负相关 (r =- 0 5 980 ,P <0 0 5 )。结论 :活动期LN患者血和尿的IL 6活性均增高 ,提示IL 6在LN的发病机制中起着一定的作用 ,血和尿的IL

Objective:To explore the action of cell factors in the pathogenesis of lupus nephritis (LN). Methods:The interleukin-6 (IL-6) activity in serum and urine in the LN active group, remissive group and the normal control group were measured by using the IL-6 dependent cell line KD83. Results:The result showed that serum and urine IL-6 activity in the LN active group was significantly higher than that of the LN remissive group and the normal control group (P<0.01). There is no significant difference on IL-6 activity in serum and urine between the LN remissive group and the normal control group (P>0.05). The IL-6 activity in serum and urine of the active LN patients was positively correlated with the level of ESR (r=0.683 0 and r=0.576 7, P<0.05) and was not correlated with the level of C3, ANA and anti-dsDNA antibody in serum. The IL-6 activity in serum of the active LN patients is positively correlated with the level of IgG in serum (r=0.835 0, P<0.05), and is negatively correlated with the level of C4 in serum (r=-0.598 0, P<0.05). Conclusion:IL-6 activity increased in serum and urine of active LN patients may play a role in the pathogenesis of LN, and it suggests that the measurement of IL-6 activity in serum and urine be a helpful indicator in monitoring the disease activity of LN.