摘要
系统地介绍了目前用于真菌电泳核型分析的真菌完整染色体DNA的 7种制备方法 ,并讨论了各种方法在真菌中应用的优缺点 ,为真菌电泳核型分析提供技术基础。
Seven different preparation methods for intact chromosome DNA of fungi used in the field of electrophoretic karyotype analysis of fungi were reviewed in this paper. Advantages and shortcomings existing in each method used in fungi were discussed, and it was advantageous to the research on the electrophoretic karyotype analysis of fungi.
出处
《食用菌学报》
2003年第2期55-59,共5页
Acta Edulis Fungi
基金
上海市科学技术发展基金资助
项目编号 (0 0 3912 0 76 )
关键词
真菌
完整染色体DNA
制备方法
电泳核型分析
Electrophoretic karyotype
Intact chromosome DNA
Protoplast method
Liquid nitrogen grounding method
Direct embeded electrophoretic method