赤潮异弯藻在铁限制条件下的光谱特性

Spectroscopic properties of Heterosigma akashiwo under iron limitation

由活体吸收光谱可见 ,赤潮异弯藻在叶绿素c靠近红光区的吸收峰处 ,由铁丰富条件下的 6 32nm向蓝漂移 2nm .由于类胡萝卜素相对于叶绿素a的比值在铁限制的细胞内增大 ,因而受铁限制的细胞活体吸收光谱在 4 80nm左右类胡萝卜素的吸收峰处增加了一个吸收峰 .赤潮异弯藻细胞低温荧光发射光谱在 6 85nm处有一明显的发射峰 ,与铁丰富条件 (10 μmol·L-1)相比 ,缺铁 (5nmol·L-1)和低铁 (10 0nmol·L-1)细胞在 6 85nm处的荧光得率分别升高了 2倍和 1.4倍 .补铁 4 8h后荧光得率则明显降低 ,表明细胞在铁限制条件下存在大量能量耗散 ,降低了细胞光合作用效率 .

Room-temperature absorption spectra of H. akashiwo cells under iron limitation showed a chlorophyll c absorption peak at 630 nm, 2 nm blue-shifted from its normal position of 632 nm. Moreover, because of the increase in the relative carotenoid abundance compared to Chl a, there was an extra shoulder peak at 480 nm for the iron-limited cells. Their fluorescence spectra (77 K) have one prominent chlorophyll emission peak at 685 nm. By comparison with Fe-replete cells (10 μmol·L -1), the fluorescence yield from 685 nm band increased by about 2 times in Fe deplete cells (5 nmol·L -1),and about 1.4 times in low iron cells (100 nmol·L -1),respectively. 48 h after Fe addition, the height of 685 nm peak was considerably decreased from that observed in low iron (100 nmol·L -1) and iron deficient (ID) cells before Fe addition,which indicated that there was a significantly higher energy dissipation, and thus,a less effective photosynthesis under the lack of Fe.