纤维细胞生长因子结合蛋白在鳞癌组织新生血管形成中的作用

Effect of FGF-BP on Angiogenesis in Squamous Cell Carcinoma

目的 研究鳞癌组织纤维细胞生长因子结合蛋白 ( FGF- BP) m RNA的表达及与癌组织新生血管形成的关系。方法 用 35S标记的 FGF- BP探针 ,原位杂交的方法检测 3 5例原发性鳞癌、8例转移性鳞癌及 7例正常鳞状上皮组织 FGF- BP m RNA的表达。样本组织中微血管采用特异性识别血管内皮细胞的抗 von Willebrand Factor单克隆抗体通过免疫组织化学证实。结果 正常肌肉、喉、舌及淋巴结组织中未发现 FGF- BP m RNA的表达 ,而鳞癌组织中 FGF- BP m RNA的表达明显增高。鳞癌组织浸润边缘的微血管密度明显增高 ,FGF- BP表达阳性的鳞癌组织中平均微血管计数较 FGF- BP阴性的鳞癌显著增高 ( P<0 .0 1)。结论 FGF- BP的表达与鳞癌微血管密度密切相关 ,提示 FGF-

Objective To localize fibroblast growth factors binding protein (FGF-BP) mRNA in squamous cell carcinoma (SCC) and study the FGF-BP association with vascularity. Methods Thirty-five primary squamous cell carcinoma specimens, 8 metastatic squamous cell carcinoma specimens and 7 histologically adjacent tissues were studied. The localization of FGF-BP mRNA expression was performed using in situ hybridization with 35 S-labeled human FGF-BP. Microvessels in tumor specimens were identified by von Willebrand Factor-specific immunohistochemical staining for endothelial cells. Results In situ hybridization demonstrated that FGF-BP mRNA was overexpressed in SCC. But a specific hybridization signal for FGF-BP mRNA was not detected in normal adult muscle, larynx, tongue, and lymph node. The highest density of microvessels was observed at the invasive edge of the tumor. The mean microvessel count in FGF-BP positive SCC was significantly higher than that in FGF-BP negative tumors (P< 0.001). Conclusion The results demonstrated that FGF-BP is present in SCC of the head and neck. The association of FGF-BP expression with angiogenesis suggests that FGF-BP may be an angiogenic factor in SCC.