对氨基苯甲酸对粘性放线菌表面疏水性的影响

Effect of Para-aminobenzonic Acid on Cell-Surface Hydrophobicity of Actinomyces viscosus

目的 研究血链球菌合成的生态调节因子——对氨基苯甲酸 ( PABA)对粘性放线菌细胞表面疏水性的影响 ,进一步了解 PABA影响细菌粘附的机制。方法 在含不同浓度 PABA( 10 - 9g/ L、10 - 7g/ L、10 - 4 g/ L)的改良Carlsson液体培养基中厌氧培养粘性放线菌 ,并采用微生物粘着碳氢化合物法 ( MATH)测试细胞表面疏水性。结果 随着培养基中 PABA浓度的增高 ,粘性放线菌细胞表面疏水性随之逐渐降低 ,其疏水率分别为 0 .3 890 0±0 .0 0 0 2 6,0 .2 5 462± 0 .0 0 168,0 .163 60± 0 .0 0 0 2 6( P均 <0 .0 5 )。结论

Objective To study the effect of Para-aminobenzonic acid(PABA) on cell-surface hydrophobicity of Actinomyces viscosus. Methods Microbial adhesion to hydrocarbons (MATH) was used to measure the cell-surface hydrophobicity of Actinomyces viscosus which grew in modified Carlsson medium with different dilution of PABA. Results Following the increase of concentration of PABA, the value of cell-surface hydrophobicity of Actinomyces viscosus decreased, which were 0.38900±0.00026, 0.25462±0.00168, 0.16360±0.00026 respectively(P<0.05). Conclusion PABA could inhibit the adherence of Actinomyces viscosus by way of changing its cell-surface hydrophobicity.