整合素β_1在新生猪肾小管上皮细胞缺氧损伤中的变化

The Function of Integrin β_1 in the Hypoxic Cellular Damage Mechanism of Renal Tubular Epithelial Cell of Newborn Porcine

目的 探讨新生儿窒息所致肾脏损害机制。方法 运用抗霉素 ( antimycin) A无糖血清的培养基 ,建立 3 0、60、90和 12 0 min肾小管上皮 ( RTE)细胞缺氧模型 ,检测 RTE细胞中三磷酸腺苷 ( ATP)含量 ,采用流式细胞技术测定 RTE细胞整合素β1 数量的变化。结果 成功建立了 3 0、60、90、12 0 min低 ATP管上皮细胞模型。各时段( 3 0、60、90和 12 0 m in)组 ATP含量较正常组明显降低 ( P<0 .0 0 1)。随缺氧时间的延长 ,整合素 β1 水平有逐渐下降趋势 ,3 0、60、90和 12 0 min组较正常组明显降低 ( P均 <0 .0 0 1) ,各组间比较 ,P均 <0 .0 0 1。结论 整合素β1 在缺氧新生猪 RTE细胞的减少 。

Objective To inquire into the mechanism of renal deficiency in neonatal asphyxia. Methods The 30, 60, 90, 120 min hypoxic renal cell model was made by use of DMEM/F-12 with antimycine A. ATP content was detected by reversed-phase HPLC.The levels of integrin β 1 of renal tubular epithelial(RTE) cell of the groups were detected using flow cytometry. Results ATP deficiency cellular model of newborn porcine RTE cell was successfully set up. The levels of ATP of the newborn porcine RET cell during 30, 60, 90, 120 min hypoxia time significantly decreased as compared with normal control (P<0.001).The levels of integrin β 1 of newborn porcine RTE cell decreased as the ATP deficiency time increased and the differences between the groups were statistically significant (P<0.001). Conclusion The decreased level of integrin β 1 in the newborn porcine RTE cell during anoxia may play an important role in the newborn renal deficiency during asphyxia.