缺血再灌注损伤对新生鼠肾小管上皮细胞细丝蛋白的影响

The Changes of Filamin Distribution in Renal Tubular Epithelial Cells During Kidney Ischemia/Reperfusion Injury in Neonatal Rats

目的 通过研究缺血再灌注不同时段细丝蛋白在肾小管上皮细胞中的分布变化 ,探讨细丝蛋白在肾脏缺血再灌注损伤中所起的作用。方法 建立新生大鼠不同时段的肾脏缺血再灌注模型 ,通过免疫荧光法检测细丝蛋白在肾小管上皮细胞上的分布及其表达量。结果 细丝蛋白在正常时主要分布在肾小管上皮细胞基底部附近 ,缺血 0 .5 h时其分布变化不明显 ,再灌注 0 .5 h后 ,细丝蛋白开始向胞浆转移 ,并出现在细胞顶部和肾小管腔内 ,再灌注 2 h这种改变最明显并伴肾小管结构的破坏 ;从再灌注 2 4h起开始进入恢复阶段 ,细丝蛋白逐渐重新回到基底部 ,再灌注 12 0 h后恢复阶段基本结束 ,肾小管结构正常。结论 细丝蛋白在缺血再灌注时分布发生改变 。

Objective By investigating the changes of filamin distribution in renal tubular epithelial cells at different ischemia/reperfusion time stages we studied the role of filamin in kidney ischemia/reperfusion injury. Methods The neonatal SD rat ischemia/reperfusion model was set up. Immunofluorescent staining was used to show the distribution of filamin in renal tubular epithelial cells and the quantities of filamin were counted by ImagePlus-Pro system. Results Filamin was located around the basal plasma membrane in normal renal tubular epithelial cells. After ischemia for 0.5 h, the redistribution was not significant. After reperfusion for 0.5 h, filamin came into the cell plasma, appeared at the apex of cells and in the lumen. This kind of change was most apparent after reperfusion for 2 h and accompanied with destruction of renal tubules. The regeneration started 24 h post-ischemia. Filamin was redistributed to the basal plasma membrane. After reperfusion for 120 h, the regeneration ended and the structure of renal tubules became normal. Conclusion The distribution of filamin was changed during ischemia/reperfusion injury, this change was prior to the change of actin cytoskeleton and integrin.