益生注射液抗移植肾缺血再灌注损伤的疗效及机理探讨

A Study on the Protective Efficacy of Yisheng Injection Against IRI in Pig Renal-Transplant Model and the Mechanism Therein Involved

目的 探讨益生注射液抗器官移植过程中缺血再灌注损伤的疗效及机理。方法 按组织配型结果将动物分成自体肾移植组和同种异体肾移植组 ,自体和同种异体肾移植组动物又再分为益生液组 (给予益生注射液 )和对照组 (不给予益生注射液 ) ,检测益生液组和对照组肾移植术前后肝、肾功能及血清中超氧化物歧化酶 ( SOD)、丙二醛 ( MDA)的含量。结果 移植前后 ,自体和同种异体肾移植组受体血清总胆红素和丙氨酸氨基转移酶均在正常范围内 ,益生液组和对照组无显著差异 ( P>0 .0 5 )。益生液组术后血清中 MDA含量升高幅度明显低于对照组( P<0 .0 5 ) ,且 SOD反应性增高水平明显高于对照组 ( P<0 .0 5 )。结论 1益生注射液可能有增强 SOD活性、减轻脂质过氧化作用 ,从而发挥其抗缺血再灌注损伤 ( IRI)作用。 2初步认为益生注射液无明显肝脏毒性 。

Objective To inquire into the mechanism of the protective efficacy of Yisheng injection against ischemia/reperfusion injury(IRI) in pig renal-transplant model. Methods A total of 24 Chinese Sichuan White Pigs (SWPs) (2-3 months old, 17.5±4.6 kg, with clear family background) were used. 6 pairs of SWPs with better results of tissue typing were allocated to the group of renal allotransplantation. The other SWPs were subjected to renal autotransplantation. Yisheng injection was added into the preservative fluid and injected intravenously after transplantion in the test groups against IRI. We detected the SWPs' liver and kidney functions and serum superoxide dismutase(SOD) and malondialdehyde(MDA) concentrations after transplantation. Results Serum creatinine and urea levels increased in 2 days after opreation and then returned to normal in both groups except the pigs suffering from acute tubular necrosis or acute rejection. The concentrations of serum MDA,SOD began to increase 2 days after operation (P<0.05). Less increase of serum MDA, less decrease of NO and more increase of SOD in test groups were detected, compared with those in control groups (P<0.05). Conclusion Yisheng injection has protective efficacy against IRI and hence may have an application foreground.