黄连素对缺血再灌注心肌细胞损伤的保护作用

Protective Effect of Berberine on Cardiac Myocyte Injured by Ischemia-Reperfusion

目的 研究黄连素对新生大鼠心肌细胞缺血再灌注损伤的保护作用。方法 取体外培养的新生大鼠心肌细胞于缺氧 2 4h复氧 1h造成缺血再灌注 ( I/ R)模型 ,观察细胞损伤情况 ;并将黄连素以 1.5× 10 - 6 m ol/ L、1.5× 10 - 5m ol/ L、1.5× 10 - 4 mol/ L 三种浓度分别加入培养基中 ,预处理 2 4h后 ,再置于上述缺氧复氧环境中培养 ,检测以上不同条件下细胞上清液中的乳酸脱氢酶 ( L DH)、丙二醛 ( MDA)、超氧化物歧化酶 ( SOD)含量 ,并检测各组细胞的凋亡率。结果 与正常对照组相比 ,缺血及再灌组细胞上清液中 L DH、MDA含量明显升高 ( P<0 .0 1) ,SOD活力则显著降低 ( P<0 .0 1) ,缺血组和再灌组凋亡率均升高明显 ( P<0 .0 1)。而用黄连素预处理后缺血及再灌组的 L DH、MDA显著低于用药前 ( P<0 .0 1) ,SOD则高于单纯缺血和再灌组 ( P<0 .0 1) ,上述作用在本实验黄连素浓度为 1.5× 10 - 6 m ol/ L～ 1.5× 10 - 4 mol/ L 范围内 ,随浓度升高而更加明显。特别是用黄连素 1.5× 10 - 5mol/ L 浓度预处理后 ,缺血组和再灌组的细胞凋亡率分别是 14.4%和 2 0 % ,分别与用药前 ( 17.4%和 41% )比较有显著性差异 ( P<0 .0 1)。结论 黄连素对缺血再灌心肌细胞有保护作用 。

Objective To observe the protective effect of berberine on cardiac myocyte injured by ischemia-reperfusion(I/R) in neonatal rats. Methods Cardiac myocytes from neonatal SD rat were cultured and pretreated with berberine in three different concentrations, 1.5×10 -6 mol/L, 1.5×10 -5 mol/L and 1.5×10 -4 mol/L, before exposure to hypoxia (95% N 2-5% CO 2) for 24 h and reoxygenation (95% air-5% CO 2) for 1 h to create cell model of ischemia-reperfusion.Then lactate dehydrogenase (LDH) release, methylenedioxyamphetamine (MDA) release ?superoxide dismutase (SOD) activity were measured, and cell apoptosis was detected. Results Compared with the measurements taken in normal group, the LDH and MDA in the supernatant of the cells in ischemia and reperfusion group increased highly (P<0.01), SOD activity decreased sharply (P<0.01), and apoptosis of cells in ischemia groups and reperfusion groups increased highly (P<0.01).Pretreatment of myocytes with berberine resulted in reduction in LDH and MDA release (P<0.01) in I/R groups,attenuation of apoptosis in ischemia and reperfusion groups (P<0.01). When the concentration of berberine was increased ,these effects were getting obvious.Especially when the myocytes were pretreated with berberine at 1.5×10 -5 mmol/L, the cell apoptosis rates were 14.4% and 20% in ischemia group and reperfusion group,which were lower than the rates of 17.4% and 41% before pretreatment, respectively. Conclusion Berberine alleviates I/R injury and attenuates apoptosis in myocytes exposed to I/R. These effects partly depend on the concentration of berberine.