高效液相色谱法测定血浆中盐酸曲马多

HPLC Method for Determination of Tramadol Hydrochloride in Human Plasma

目的 建立反相高效液相色谱 -紫外检测法检测盐酸曲马多浓度的方法。方法 血样预处理采用两次液 -液反萃法。采用 ODS色谱柱 ( 15 0× 4.6mm) ;流动相为乙腈∶磷酸盐缓冲溶液 ( 0 .0 2 mol/ L,p H3 .7) =17∶83 ( V/ V) ;流速 :1.0 ml/ min;检测波长 :λ=2 16nm。结果 血浆中盐酸曲马多的最低检测浓度为 10 .2 ng/ m l,在2 5～ 80 0 ng/ ml的浓度范围内线性关系良好。结论 该法结果准确 ,重现性好 。

Objective A reversed-phase high performance liquid chromatography(HPLC)method was established to determine the concentration of tramadol hydrochloride in human plasma. Methods HPLC instrument was used with the column:150 mm×4.6 mm(Li Chrosorb C 18, 5 μm). The mobile phase was composed of phosphate buffer solution (0.02 mol/L,pH=3.7)-acetamide (83∶17,V/V). Flow rate was 1.0 ml/min. Detection wavelength was 216 nm. Results The standard curve equation was Y=0.03244X+0.6007.The linear range was 25-800 ng/ml. The minimum detection limit was 10.2 ng/ml. Conclusion The reversed-phase HPLC method is simple, rapid and sensitive. It is applicable to the determination of the concentration of tramadol hydrochloride in human plasma.