海洋放线菌胞外多糖发酵工艺及其条件研究

A STUDY ON THE FERMENTATION TECHNOLOGY FOR A MARINE ACTINOMYCES EXTRACELLULAR POLYSAC-CHARIDE

以具有明显海洋特性的、产胞外免疫活性多糖的链霉菌2305菌株作为发酵菌种,对其胞外多糖的发酵工艺及其条件进行了研究,考察了不同碳源、氮源、水质、培养基初始pH值、发酵温度、通气量等因素对发酵菌种的生物量及胞外多糖产量等的影响,建立和优化了胞外多糖摇瓶发酵培养基配方、发酵工艺及发酵条件,胞外多糖产量达到4.58g/L,转化率达到6.90％。研究了发酵过程中菌体生物量、底物糖残余量、pH值、产物多糖积累量等的动态变化,分析讨论了2305菌株胞外多糖发酵的动力学类型。

Using the strain of marine actincmyces 2305, which produces extracellular polysaccharides(EPS) with immunomodu-lating activity, as a fermentative strain, the fermentation process and the fermentation conditions for the EPS are studied. A suitable medium and a suitable shaking culture condition are established, and the yield of the EPS reaches 4.58 g/L. The changes of the biomass yield, residual glucose concentration, pH, productivity of the EPS through the fermentation course are studied, and the fermentation kinetic type for the EPS is discussed.