SEA诱导人膀胱癌BIU-87细胞凋亡机制的研究

Study of apoptosis mechanism of the carcinoma of urinary bladder BIU-87 cell strain induced by SEA

目的 :为了观察葡萄球菌肠毒素A(SEA)对人膀胱癌BIU 87细胞株诱导凋亡机制。方法 :应用MTT比色法和流式细胞术检测SEA对BIU 87细胞增殖抑制率和对细胞周期的影响。结果 :SEA对BIU 87细胞株有明显的抑制作用 ,且呈剂量依赖性。流式细胞术分析 ,SEA能阻止G1 期细胞向S期的进程 ,G1 期细胞堆积。结论 :SEA能诱导BIU 87细胞凋亡 ,具有细胞毒性效应 ,可用于膀胱内灌注 ,从而抑制膀胱癌的浸润转移。

Objective:Observing the mechanism of apoptosis in the carcinoma of urinary bladder BIU 87 cell strain induced by staphylococcus enterotoxin A (SEA).Methods:The multiplicative inhibitory rate of the BIU 87 cell strain and cell cycle affected by SEA was tested with MTT colorimetry and low cytometry.Results:SEA restrain obviously the BIU 87 cell strain and have dosage dependence and obstruct the cell multiplication processes from G 1 period to S period.A crowd of cells was seen G 1 period.Conclusion:SEA having effect of cytotoxicity can make BIU 87 cell strain apoptosis and prevent the metastsis of the carcinoma of urinary bladder by pouring it into bladder.