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藏猪白细胞介素4基因cDNA的克隆及序列分析 被引量：5

Cloning and analyzing of interleukin-4 cDNA from the lymphocyte of Tibet pig
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摘要 目的 :克隆藏猪白细胞介素 4基因cDNA。方法 :从体外ConA刺激 70小时的藏猪外周血淋巴细胞中提取总RNA ,应用RT PCR技术扩增 ,pMD T载体连接 ,常规转化后 ,进行酶切及序列测定进行鉴定。结果 :研究表明克隆得到的IL 4基因cDNA与成华猪的IL 4同源性达到 99% ,与长白杂交猪的同源率为 98%。结论 :从藏猪外周血淋巴细胞中成功地分离到IL 4基因。 Objective:To clone interleukin 4 cDNA of Tibet pig.Methods:To amplify cDNA,using RT PCR from the total RNA from the lymphocyte stimulated by 10 μg/ml Con A in vitro,to subclone into pMD T vector,and then to determine it by digesting and sequencing.Results:The cloned Tibet pig interleukin 4 was 98% homologus to the necleotide acids of Chenghua pig a special specie of pig in Sichuan province and 99% homologus to that of hybrid of Landrance with Chanbai pig.Conclusion:IL 4 of Tibet pig was successfully cloned from the lymphocyte after stimulating 70 h with ConA.
作者 李惠 高荣 武梅 王丽焕 丁晓涛 李平 李波 余庆 郑勇 刘崑 刘世贵
机构地区 四川大学草原生物防治工程国家专业实验室 四川省甘孜州畜牧科学研究所
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2003年第7期475-478,共4页 Chinese Journal of Immunology
基金 国家自然科学基金课题 ( 3 0 170 70 3 )资助
关键词 藏猪 白细胞介素4 基因CDNA 克隆 序列分析 Tibet pig Interleukin 4 gene Cloning Sequence analysis
分类号 R392.1 [医药卫生—免疫学]
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参考文献13

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二级参考文献1

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共引文献2

同被引文献48

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引证文献5

二级引证文献16

中国免疫学杂志
2003年 第7期

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