脂质体介导IL-2、TNF-α联合基因转导体内抗肝细胞癌的实验研究

Combined IL-2 cDNA with TNF-α cDNA genetherapy for hepatocellular carcinoma

目的 :探讨脂质体介导IL 2cDNA、TNF αcDNA联合基因转导体内抗肿瘤效果。方法 :建立裸鼠肝癌动物模型 ,在荷瘤部位将脂质体包裹的IL 2cDNA和TNF αcDNA直接注入瘤体中 ,观察肿瘤大小变化 ,并检测此 2种基因在肿瘤组织中的表达情况。结果 :IL 2基因和TNF α基因联合治疗组肿瘤生长明显受到抑制 ,抗肿瘤效果显著优于单纯治疗组和对照组。结论 :IL 2和TNF α基因联合治疗组对肿瘤生长抑制效果明显优于单纯治疗组与对照组 ,荷瘤鼠生存期明显延长 (P <0 0 5 )。

Objective:To explore antitumor effects of combined interleukin 2(IL 2)gene and tumor necrosis factor(TNF α)gene therapy in nude mice model with hepatocellular carcinoma.Methods:Nude mice model with hepatocellualr carcinoma was established with BelH7402 cell line.Plasmid vector was used to transfect BelH7402 and nude mice model with the gene for human IL 2 or TNF α alone or in combination by lipofectamine reagent and tumor size in nude mice model with BelH7402 carcinoma was measured before and after treatment to evaluate the response of the different treatment and control groups.Results:Tumor and BelH7402 transfected with IL 2 or TNF α gene by liposome mediated were successful.Growth rates of BelH7402 transfected with IL 2 or TNF α gene were not significantly changed,growth rate of tumor in nude mice model was significantly inhibited in combined IL 2 and TNF α genetherapy group as compared with the other groups(P<0 05).Conclusion:Combination of the two genes in genetherapy may be additive of synergistic in antitumor effect.