五加皮抗肿瘤活性物质Age对单核细胞产生TNF-α和IL-12的影响

Stimulating activity of Acanthopanax gracilistylus anti-tumor component on monocytes

目的 :研究五加皮抗肿瘤成分Age对单核细胞功能的影响 ,揭示Age抗肿瘤机制。方法 :使用对TNF敏感的L92 9细胞采用生物法测定了TNF含量 ,用ELISA法测定了IL 12的含量。用RT PCR法分析了单核细胞的细胞因子mRNA表达。用同位素法分析了Age对单核细胞吞噬功能的作用。结果 :Age与单核细胞共同培养后 ,单核细胞TNF α、IL 12等细胞因子的产生明显增加 (P <0 0 1) ,而且有较好的量效关系。在单核细胞与LPS的培养液中加入不同浓度的Age ,经培养后与单独加入LPS相比TNF α分泌的量显著增加 ,显示了Age对LPS的单核细胞刺激有相加作用。经Age处理后 ,单核细胞的TNF α、IL 12等细胞因子的mRNA表达也明显增强。体内实验结果也显示 ,Age对单核细胞的细胞因子产生有一定的影响。荷瘤小鼠经口连续投入Age后 ,血清中和脾脏分离的单核细胞体外培养上清中TNF α和IL 12含量明显提高 ,单核细胞TNF α、IL 12mR NA表达也明显增强 (P <0 0 1) ,从蛋白水平和分子水平证明了Age对单核细胞的细胞因子产生有促进作用。结论 :中药五加皮抗肿瘤有效成分Age对单核细胞有较强的刺激作用。通过促进其细胞因子的分泌和吞噬功能的增强杀伤肿瘤或抵抗肿瘤发生 ,所分泌的细胞因子还可通过调节免疫功能达到治疗肿瘤作用。该结果对五加皮?

Objective:Study the effect of Acanthopanax gracilistylus extract(Age) on monocyte,and reveal the anti trmor mechanism.Methods:The content of TNF α,IL 12 were examined by using biological method and ELISA,respectively.The expression of monocytic cytokine mRNA are investigated by RT PCR.The effect of Age on the phagocytosis of monocytes is studied using TdR incorporation method.Results:After cultured with Age,the production of cytokines by monocytes such as TNF α,IL 12 are markedly increased with good dose dependent manner.After monocytes cultured with LPS in the presence or absence of Age,Age markedly enhances LPS induced TNF α production.Treatment with Age,the production of cytokines and the expression of their mRNA are increased simultaneously.The results in vivo also show:Age can enhance the productions and the expressions of TNF α and IL 12.Conclusion:The anti tumor active component of Age has obvious stimulative activity on monocytes, increasing the production of cytokines, enhancing the phagocytosis.These results product the scientific evidence for the study of anti tumor mechanism and clinical ultilization.