牛磺酸和支链氨基酸对大鼠肝脏纤维化模型形态影响

EFFECTS OF TAURINE AND BRANCHED CHAIN AMINO ACID ON MORPHOLOGY OF RAT HEPATIC FIBROSIS MODEL

①目的 探讨牛磺酸和支链氨基酸 (T&B)对大鼠肝脏纤维化模型肝脏形态的影响。②方法 用体积分数 0 .6的CCl4橄榄油溶液 3mL/kg体质量背部皮下注射制备大鼠肝纤维化模型 ,并饲一定比例混合的T&B复合物。实验分为对照组、模型组、甘利欣 (DGI)组和T&B(0 .5、1 .0、2 .0g/kg体质量 )组。 1 2周后观察大鼠肝组织的病理变化 ,并应用免疫组化技术及图像分析技术检测Ⅰ、Ⅲ型胶原在肝组织中的分布及含量。③结果 模型组肝组织纤维化程度与对照组相比有显著差异 (H =33.6 39,q =7.4 2 4 ,P <0 .0 1 )。应用T&B 1 .0、2 .0 g/kg体质量 6周后 ,Ⅰ、Ⅲ型胶原含量均明显下降 ,与模型组相比亦有显著差异 (Ⅰ型 :F =1 6 .4 35 ,q =8.739、8.378,P <0 .0 1 ;Ⅲ型 :F =1 0 .6 6 9,q =7.0 88、7.792 ,P <0 .0 1 )。 ④结论 T&B能够影响肝组织中Ⅰ、Ⅲ型胶原等细胞外基质的沉积 。

Objective\ To study the influence of taurine and branched chain amino acid (T&B) on the morphology of rat hepatic fibrosis model. \ Methods\ A hepatic fibrosis model was induced by injecting 60% carbon tetrachloride (CCl 4), 3 mL/kg body weight, subcutaneously. Experimental rats were divided into control, model, DGI, T&B( 0.5, 1.0, 2.0 g/kg) groups and fed on a compound commixed with taurine and branched chain amino acid in the optimal proportion. The pathologic alteration of liver tissue was observed 12 weeks later and the distribution and content of collagen type Ⅰ and Ⅲ in the liver tissue were analyzed by immunohistochemistry and imaging analysis system.\ Results\ The degree of hepatic fibrosis was much more serious in the model group than that in the control group ( H=33.639,q=7.424,P <0.01). After T&B 1.0 or 2.0 g/kg body weight, was used for 6 weeks, the content of col Ⅰ and col Ⅲ were much lower than those in the model group (typeⅠ: F=16.435, q=8.739, 8.378, P < 0.01; type Ⅲ: F=10.669, q=7.088, 7.792, P <0.01). \ Conclusion\ Taurine and branched chain amino acid can delay the development of hepatic fibrosis induced by CCl 4 and its possible mechanism is to inhibit the accumulation of extracellular matrix such as col Ⅰ, Ⅲ.