SARS相关冠状病毒S2基因的克隆及其DNA疫苗的免疫效果

Cloning of SARS coronavirus S2 gene and immunization effect of its DNA vaccine

目的 :构建抗原基因为 SARS相关冠状病毒 (severe acute respiratory syndrome coronavirus,SARS- Co V) S2基因的DNA疫苗 ,将其接种小鼠后观察病毒特异性抗体产生情况。方法 :将合成的 S2基因片段克隆至真核表达载体 ,随之将质粒进行小鼠肌内接种免疫 ,定期检测血清中抗 SARS- Co V的病毒特异性抗体水平。结果 :疫苗接种后第 2周就能检测出病毒特异性抗体 ,随着时间的延续 ,抗体水平逐步升高 ,而空质粒对照组未检测出明显的特异性抗体。 结论 :以 S2为抗原基因的 DNA疫苗能诱导

Objective: To construct DNA vaccine plasmid against S2 gene of SARS coronavirus(SARS CoV) and to measure the SARS CoV specific antibodies in mice inoculated with the plasmid. Methods:The synthesized DNA encoding S2 fragment was inserted into eukaryotic expression vector, then the recombinant plasmid DNA was directly injected into BALB/c mice intramusclarly.ELISA was used to measure SARS CoV specific antibodies in mice. Results: Specific antibodies was induced 2 weeks after immunization and showed a rising trend as time went by. Conclusion: S2 can be used as an antigen of DNA vaccine to induce SARS CoV specific antibodies.