SARS相关冠状病毒M基因片段的克隆及其DNA疫苗诱导的体液免疫

Cloning of SARS coronavirus M gene and humoral immune reaction induced by its DNA vaccine

目的 :构建含有 SARS相关冠状病毒 (severe acute respiratory syndrom e coronavirus,SARS- Co V) M基因片段的核酸疫苗 ,观察其免疫小鼠后病毒特异性抗体产生情况。方法 :将合成的 M基因片段克隆到核酸疫苗真核表达载体 p VAX1中 ,免疫小鼠后 ,用 SARS- Co V抗体 EL ISA检测试剂盒定期检测免疫小鼠血清中抗 SARS- Co V的病毒特异性抗体水平。 结果 :构建了重组核酸疫苗质粒 p VCo- M,免疫小鼠后可诱导产生出病毒特异性抗体。 结论 :以 M为抗原基因的 DNA疫苗能诱导SARS病毒特异性抗体 ,为进一步改进或探索同类 DNA疫苗提供有益启示。

Objective:To construct DNA vaccine containing SARS CoV M gene and to observe the production of SARS CoV specific antibodies in mice immunized with the plasmid. Methods: A recombinant DNA vaccine plasmid was constructed by cloning SARS CoV M gene fragment into pVAX1.After mice immunized with the recombinant DNA vaccine, the SARS CoV antibodies in the sera of mice were detected with ELISA kit. Results: A recombinant plasmid pVCo M was constructed which could induce SARS CoV specific antibodies in mice. Conclusion: M gene of SARS CoV can be used as an antigen of DNA vaccine to induce SARS CoV specific antibodies.