摘要
取重组人表皮生长因子 (rh EGF)分别加入到人工胃液 (p H2 )、人工肠液 (p H6 .8)和加胃蛋白酶的人工胃液、加胰酶的人工肠液中 ,定时取样 ,采用放射免疫分析法测定不同时间内 rh EGF含量。结果发现 ,rh EGF在人工胃液和人工肠液中的含量随时间的延长而呈下降趋势 ,1h后人工胃液和人工肠液中 rh EGF浓度分别下降 4 3.4 6 %和 2 1.91% ;但在酶的存在下1h后 rh EGF浓度分别下降 89.6 2 %和 79.5 2 %。这表明 rh EGF在人工胃、肠液中均比较稳定 ,但在酶的存在下 rh
This paper is to study the stability of recombinant human epidermal growth factor (rhEGF) in artificial intestinal juice and gastric juice,providing references for its clinical application in intestinal and stomach injury.RhEGF was added into artificial intestinal juice and gastric juice respectively, and the content of rhEGF was determined with RIA at preset time points. The content of rhEGF in artificial gastric juice (pH 2) and intestinal juice (pH 6.8) had a tendency to decrease as time went by,reaching 43.46% and 21.91% from their baselines 1 h later respectively. But the decrease of rhEGF reached 89.62% and 79.52% 1 h after the presence of peptic enzymes respectively. The rhEGF in the artificial intestinal juice and gastric juice was relatively stable but the process of its inactivation could be greatly expedited in the presence of peptic enzymes.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2003年第7期810-811,共2页
Academic Journal of Second Military Medical University
基金
国家重大基础研究规划资助项目 ( 19990 5 42 0 4)
关键词
肠液
人工胃液
重组人表皮生长因子
稳定性
胃蛋白酶
recombinant human epidermal growth factor
artificial intestinal juice
artificial gastric juice
stability
peptic enzymes