C.pasteurianum高产1,3-丙二醇菌株的选育及发酵培养基的研究(Ⅰ)

The Screening of the 1,3-PD High-production Strain from Clostriduims and Studies on Its Fermentation Medium

以淡水湖泊泥土中分离出的 13株梭菌 (Clostridium)为出发菌株 ,利用常规筛选方法选出 2株 1,3 丙二醇产生菌 (Clostridiumpasteurianum)。经UV、DES、NTG、EMS、LiCl单独及复合诱变 ,选育出一株 (CpN 38) 1,3 PD高产突变株。该突变株较出发菌株 ( 98- 77,98- 10 8) 1,3 PD产量提高了 7倍 ,产量为 31.0 0g/L。通过单因素实验 ,确定了CpN 38发酵培养基为 :甘油 ,90g/L ;NH4Cl,1.70g/L ;Fe2 +,0 .0 0 5 % ;Co2 +,0 .0 0 4 %。

Two 1,3 PD producing strains were obtained from 13 Clostridiums, which were isolated from freshwater marsh. A 1,3 PD producing mutant(CpN 38)was bred through multiple mutagenesis (UV, DES, NTG, EMS, LiCl etc).The yield of 1,3 PD was 31.00*#g/L, which was 7 times of original strain (Clostridium pasteurianum 98-107 and 99-77). Through monofactorial experiment, the optimal medium was obtained for producing 1,3 PD of CpN 38.The medium was 90*#g/L glycerol, 1.70*#g/L NH 4Cl, Fe 2+ 0.005%, Co 2+ 0.004 %.