Streptococcus zooepidemicus H23生产透明质酸营养条件的优化

Optimization of Nutritional Conditions on the Production of Hyaluronic Acid by Streptococcus zooepidemicus H23

在发酵罐水平上研究了酵母粉用量、初糖浓度和碳氮比以及补加葡萄糖对Strepto coccuszoopeidemicusH2 3发酵生产透明质酸的影响。利用上述条件在 5L罐上进行发酵实验 ,16h时发酵液中透明质酸质量浓度为 4 83g/L。

The effects of some nutritional conditions,such as yeast extract concentration,feeding glucose as well as ratio of C/N in batch culture,on the production of hyaluronic acid by Streptococcus zooepidemicus H23 were investigated In 5 L jar fermentor batch culture:(1)more than 20*#g/L of yeast extract inhibited the accumulation of HA;(2)production of HA was increased from 4 338*#g/L to 4 837g/L by simply feeding glucose;(3)increasing the concentration of glucose and yeast extract proportionally with constant C/N ratio(12∶1) improved the production of HA It was also found that,glucose consumption and HA production almost stopped under the condition of nitrogen difficiency