体外大鼠肝细胞中毒模型的建立与初步应用

THE ESTABLISHMENT AND PRELIMINARY USE OF D-GALACTOSAMINE CYTOTO- XICITY MODEL OF PRIMARILY CULTURED HEPATOCYTES

以D-氨基半乳糖(D-Gal)作用于原代培养的大鼠肝细胞,通过检测培养细胞上清中丙氨酸转氨酶(ALT)值,建立了体外肝细胞中毒模型。利用此模型,初步探讨了肝再生刺激物质(HSS)、强力宁及肝安的体外降酶作用。实验发现,HSS虽能刺激正常大鼠肝细胞DNA合成增加,但在体外单独使用却未能发现其具有降酶作用;强力宁及肝安在一定剂量范围内能降低ALT值。由此认为,该模型可作为初步筛选降酶药物的一种较为直观的方法。

A cytotoxicity model of primary cultured hepatocytes with D-galactosamine (D-Gal) has been established. The ALT value of culture medium was used as the marker of cell injury. Using this model, the in vitro antihepatotoxic effects of hepatic stimulator substance (HSS), isolated from regenerating adult rat liver, as well as Potenlin and 15-amino acid solution were observed. The results showed that although HSS could stimulate DNA synthesis in serum-free culture of adult rat hepatocytes, it had no effect to reduce ALT value in vitro. However the latter could be reduced by Potenlin or 15-amino acid solution in high concentrations. The described model may be useful for preliminary screening of antihepatotoxic activity of drugs.