胃粘膜活检粘液组化染色法的选择与改进

SELECTION AND IMPROVEMENT OF HISTOCHEMICAL STAINING TECHNIC OF MUCIN FOR GASTRIC MUCOSA BIOPSY SPECIMEN

作者对比了12种粘液组化染色方法,选择奥新蓝pH2.5/过碘酸雪夫反应(AB pH2.5/PAS),奥新蓝pH1.0/苦味酸酸性复红(AB pH1.0/VG)为一组粘液组化方法。改进了染液配制:(1)在失效的雪夫试剂中加适量偏重亚硫酸氢钠可使其恢复染色效果。(2)减1％奥新蓝为0.5％。(3)改中性红对比染色为VG对比染色。使用此组粘液组化方法,结果鲜明、稳定、特异,操作简便,价格低廉。适合于各级医院的病理科对胃粘膜活检组织作常规检测。

Twelve methods of histochemical staining of mucins were compared,and improvemen ts in staining technic was made. Appropriate sodium meta-bisulfite was added to the ineffective Schiff's reagent to recover its staining property. The concentration of alcian blue was decreased from 1 % to 0.5%. Neutral red as the contrast stain was replaced by VG. The result of this study showed that this series of staining technics was simple to carry out, low cost, more specific, and suitable for any pathology department to use for detection of mucin in biopsy specimens of gastric mucosa.