婴儿肝炎和肾病综合征患儿尿标本中巨细胞病毒DNA的检出

DETECTION OF HUMAN CYTOMEGALOVIRUS DNA IN URINE SPECIMENS FROM CHILDREN WITH HEPATITIS OR NEPHROPATHY SYNDROME

建立了聚合酶链反应(PCR)检测人巨细胞病毒(HCMV)DNA的方法,证明其高度特异性和敏感性。HCMV-AD_(169)株DNA的E_(co)R_1酶切J片段最小检出量为0.1fg,相当于6个基因拷贝,比地高辛标记探针的DNA杂交试验的敏感度(1pg)高10 000倍。用PCR技术检测了63份婴儿肝炎患儿和41份肾病性综合征患儿尿标本和部分肝穿刺标本,HCMV-DNA的高阳性率检出结果表明,HCMV是引起婴儿肝炎的重要病原。肾病性综合征的病因可能也与HCMV感染有关。

The polymerase chain reaction (PCR) for detection of human cytomegalovirus (HCMV) was developed and the specificity and sensitivity of PCR was proved. The lowest detection level was 0.1 fg of HCMV-AD169 strain DNA-EcoRl-J-fragrnent, corresponding to approximately 6 gene copies.It was 1 000 times as high as the sensitivity of DNA hybridization with Digoxigenin labelling probe. The urine specimens from pediatric patients with hepatitis or nephropathy syndrome were examined by PCR. High HCMV-DNA positive rates indicated that HCMV was the important pathogen of the pediatric patients with hepatitis, and that nephropathy syndrome may be related to HCMV.