脑损伤后脑与血中钙和钙调素含量变化及氢溴酸山莨菪碱对其影响

EFFECT OF ANISODAMINE ON CHANGES IN CALCIUM AND CALMODULIN CONTENTS IN THE BRAIN TISSUE AND THE BLOOD FOLLOWING BRAIN INJURY

采用大鼠脑落体损伤模型,分别检测脑损伤后不同时间脑组织水含量、脑皮质和血中钙及钙调素(CaM)含量,并应用氢溴酸山莨菪碱治疗。结果:(1)脑损伤后脑及血中钙含量均有增高,以脑皮质钙含量增高较为明显,伤后48h达24.12±10.22mmol/kg干脑,约为对照值的2倍;(2)脑皮质及血中CaM含量均有较明显升高,伤后48h达峰值,分别为对照值的2.6倍和2.8倍;(3)脑损伤后脑白质水含量显著增加,其变化规律与脑组织中钙及CaM相一致;(4)应用氢溴酸山莨菪碱后钙及CaM含量显著降低,脑水肿明显减轻。

The changes in calcium, calmodulin (CaM) and water contents of brain tissues were studied in the rat after brain injury, which was induced by a falling weight. The effect of anisoda-mine (654-2) on changes in calcium, CaM contents and brain edema were investigated. The results showed that: (0 contents of calcium in the brain cortex and serum were increased after brain trauma, and alteration in calcium in the brain cortex was more prominent than that of serum. It reached 24.12±10.22 mmol/kg dry brain 48h after injury, which was two times of control values at that time; (2) the augmentation of CaM in the brain cortex and plasma was remarkable. It reached its peaks at 48h, and its values were 2.6 to 2.8 times of control values respectively; (3) the increment of water content in the white matter was remarkable, which was closely correlated to the changes of calcium and CaM; (4) the injured animals that received anisodamine showed reduction in the levels of calcium and CaM distinctively, and the degree of brain edema was less.