灰葡萄孢菌nit突变体的诱导及其在营养体亲和性测定中的应用初探

Induced nitrate non-utilizing mutants of Botrytis cinerea and their use in vegetative compatibility tests

从山西运城、临汾、长治、晋中、大同等地保护地黄瓜灰霉病病株上采集、分离的分属于 3个不同菌丝融合群的 8个灰葡萄孢菌单孢菌株 ,经氯酸盐诱导处理 ,共获得了抗氯酸盐的硝酸盐利用缺陷突变体 (nit突变体 ) 5 9株 ,其中nit1型 38株 ,nit3型 10株 ,nitM型 11株。所有nit突变株分别在PDA斜面转管培养 3次 (2 1d)后 ,除 6株恢复成野生菌株外 ,其余多数nit突变菌株表现稳定。来源于同一野生菌株的不同类型nit突变体间或同一菌丝融合群不同野生菌株的nit突变体间可产生互补反应而形成异核体 ,其中以nitM型突变株互补性最好 ,在利用nit突变体测定灰葡萄孢菌营养体亲和性时应作为标准菌株。来源于不同菌丝融合群的nit突变体间不能产生互补反应。

Eight single spore isolates of Botrytis cinerea that belong to three different anastomosis groups were obtained from grey mould cucumber plants collected from Yuncheng, Linfen, Changzhi, Jinzhong, Datong in Shanxi Province. All of the 8 isolates produced chlorate resistant sector on MMC media and nitrate non utilizing mutants ( nit mutant),which were recovered by transferring the chlorate resistant sectors to the MM media containing nitrate as single nitrogen source. Among the 59 nit mutants, 38 nit mutants were nit1 ,10 nit mutants were nit3 ,and 11 nit mutants were nitM . Majority of nit mutants were stable. Complementation occurred between the different nit mutant phenotypes derived from the same parental strain or different strain of the same anastomosis group. Complementation occurred readily and reliably in nitM which was used as the tester to identify the vegetative compatibility group to which isolates B.cinerea belongs. No complementation was observed between any nit mutants recovered from the strains of different anastomosis groups.