粉防己碱对烟雾吸入伤家兔炎症细胞合成释放白三烯B_4的影响

THE EFFECT OF TETRANDRINE ON THE RELEASE OF LEUKOTRIENE B_4 BY INFLAMMATORY CELLS OF RABBITS WITH SMOKE INHALATION INJURY

利用重度家兔烟雾吸入伤模型,观察了66只家兔(正常对照组6只,致伤对照组30只和粉防己碱治疗组30只)血中性粒细胞和肺泡巨噬细胞合成释放白三烯B_4(LTB_4)的动态变化。结果伤后72h内,中性粒细胞LTB_4合成释放显著增加(P<0.05、0.01);伤后24h内,肺泡巨噬细胞LTB_4合成释放也明显增加(P<0.05、0.01)。粉防己碱可以减少支气管肺泡灌洗液中白细胞数,降低伤后24h的兔肺及气管微血管通透性,抑制中性粒细胞及肺泡巨噬细胞LTB_4的生物合成。提示LTB_4与吸入伤气道及肺损害有关,粉防己碱可以通过抑制炎症细胞合成释放LTB_4,从而减轻吸入伤。

A rabbit model with severe smoke inhalation injury was adopted. 66 rabbits were divided into three groups---normal control (6), injury control (30) and tetrandrine treatment (30) . Thedynamic changes in leukotriene B4 (LTB4) release by blood neutrophils (Neu) and alveolar macrophages (AM) were observed in the first 7 d post injury. It was found that the synthesis and release of LTB4 by Neu was elevated significantly in 72 h post injury, and also by AM in 24 h. Tetrandrine could decrease the number of white blood cell in bronchoalveolar lavage fluid, suppress the increase in tra-cheal and pulmonary vascular permeability, inhibit the synthesis and release of LTB4 by Neu and AM. These results showed that there was a close relationship between the release of LTB4 and inhalation injury, and tetrandrine could reduce the injury probably through the inhibition of the release of LTB4 by inflammatory cells.