球花石楠离体培养及快速繁殖研究

RAPID PROPAGATION OF PHOTINIA GLOMERATA IN VITRO

取球花石楠的不同器官、不同部位的材料进行离体培养及快速繁殖研究。试验结果表明 ,根部萌蘖条和盆栽大苗的嫩茎为首选外植体 ,在MS + 6 BA 2mg·L- 1 +NAA 0 .5mg·L- 1的培养基上有较高的腋芽萌发率 ,增殖阶段以MS + 6 BA 4mg·L- 1 +NAA 0 .1mg·L- 1 培养基上的小苗最多 ,生长健壮 ,生根可在 1 /2MS +IBA 0 .2 5mg·L- 1 的培养基上进行。分析了生根较低的原因并提出改进措施。

Rapid propagation of different parts from different organs of Photinia glomerata in vitro was studied. The results showed that the high germination rate of axially bud from the explants of stump shoot and potted seedling appears on the medium of MS+6-BA2 mg·L -1+NAA 0.5mg·L -1. The most numerous and sturdy plantlet appears on the medium of MS+6-BA4mg ·L -1+NAA 0.1mg· L -1during breeding. The roots grow well on the medium of 1/2 MS+IBA 0.25mg·L -1. The reasons of lower strike root rate were discussed and the improved measures were put forward.