东北延胡索愈伤组织培养及延胡索乙素形成的适宜条件

CALLUS CULTURE AND CONDITIONS FOR DL-TETRAHYDROPALMATINE FORMATIONIN CORYDALIS YANHUSO FROM NORTHEAST OF CHINA

比较了东北产两种延胡索各器官的愈伤组织发生情况 ,采用单因子和正交设计试验 ,对齿瓣延胡索 (Corydalisremota)愈伤组织生长及延胡索乙素形成的适宜条件做了研究。结果表明 ,齿瓣延胡索各器官对培养基的响应性均显著高于东北延胡索 (C .ambigua) ,其块茎愈伤诱导率高达 93.3% ;MS培养基对于愈伤组织诱导较White有利 ,2mg·L- 1 2 ,4 D与 1mg·L- 1 KT的组合愈伤诱导率最高 ,并适于继代培养。正交试验结果表明 ,愈伤组织生长和延胡索乙素形成的适宜条件存在差异 ,在 2 7组处理中 ,1号培养基有利于培养物鲜重的提高 ,9号有利于干物质的积累 ,1 0号则有利于的延胡索乙素形成 ;培养基中几种无机离子和有机成分均显著影响愈伤组织生长和延胡索乙素形成 ,NH4+/NO3- 与Ca2 +和K+之间的交互作用对于生物量积累和延胡索乙素含量均有显著影响。

Formation status of callus from different organs were compared between the two species of Corydalis Yanhuso from Northeast area. Suitable medium for callus growth and formation of dl-tetrohydropalmatine(THP) of C. remota were studied employing monofactorial experiment and orthogonal design. The results showed that responsibility among different organs of C. remota to media was stronger than that of C.ambigua, and induce rate of callus from stem tuber was 93.3% high. MS medium was suitable for callus growth, and inductivity of callus in the combination of 2 mg/L 2,4-D and 1mg/L KT was the highest among the tests, and the callus was suitable for subculture. The experiment of orthogonal design indicated that there was difference of suitable culture condition between growth of callus and formation of THP. In 27 different media,No.1was the best for increasing fresh weight of callus, and No.9 was the best for accumulation of dry matter, while No.10 medium was the best for formation of THP. Either inorganic ions or organic components were the factors significantly affecting growth of callus formation of THP. Biomass accumulation and THP content were all affected by the mutual effects of NH 4+/NO 3-with either Ca 2+ or K+ .