大鼠睾丸Sertoli细胞内微管分布的研究

DISTRIBUTION OF MICROTUBULES IN SERTOLI CELL OF RAT TESTIS

本研究应用免疫荧光和透射电镜方法,对大鼠睾丸Setroli细胞内微管的分布进行了观察。结果表明,Serto|i细胞内微管主要分布于核上方胞质内,沿细胞长轴排列,并随Sertoli细胞主干及分支伸展于各生精细胞之间,直至上皮顶部。在生精上皮周期中,伴随上皮构筑的改变及Sertoli细胞的变形,微管分布呈现规律性变化。Ⅰ～Ⅴ期长形精子细胞周围微管密集,沿细胞长轴顺行。Ⅶ期时,富含微管Sertoli细胞突起包绕于精子细胞头部周围,并将其送入和悬吊子曲管腔内,部分微管伸展方向与精子细胞头部镰状外形一致。精子释放后(Ⅷ～Ⅸ期),Sertoli细胞突起及其微管回缩;在开始伸长变形的精子细胞周围微管再次聚集。本研究结果提示,Sertoli细胞内微管分布及其规律性变化与生精上皮构筑,Sertoli细胞外形变化及精子细胞的运动和释放密切相关。

The distribution of microtubules (MTs)in the Sertoli cell of rat testis was studied with indirect immunofluorescence and electron microscopy. We have found that MTs are mainly located in the cytoplasm apical to the nucleus and oriented parallel to the long axis of the Sertoli cell. MTs may extend into the stalks and processes of the cell which embrace different germ cells. With the changes of the architecture of the seminiferous epithelium and the shape of Sertoli cells, there are some regular changes in MTs distribution during the seminifeous epithelium cycle. In stage Ⅰ-Ⅴ, many MTs aggregate around the elongated spermatids and are parallel to their long axis. During stage Ⅶ maturing spermatids are suspended into the lumen by the Sertoli cell processes containing numerous MTs. Some of the MTs conform to the contour of the hook-shaped spermatids heads. After spermiation (stage Ⅶ-Ⅸ), MTs retract from the lumen with the Sertoli cell processes and gather around the spermatids which just start their elongation. These results indicate that the distribution of the MTs in Sertoli cell has close relations with the architecture of the seminiferous epithelium, and the changes of the Sertoli cell shape and the movement of the spermatids.