大鼠延髓腹外侧区含酪氨酸羟化酶、神经降压素和胆囊收缩素样的神经元向伏核的投射—HRP和免疫细胞化学双重标记法研究

THE PROJECTION OF THE TYROSINE-HYDROXYLASE (TH), NEUROTENSIN (NT), CHOLECYSTOKININ (CCK) CONTAINING NEURONS IN THE VENTROMEDIAL MEDULLA TO THE NUCLEUS ACCUMBENS IN THE RAT——A STUDY BY COMBINED HRP RETROGRADE TRACING AND IMMUNOCYTOCHEMICAL METHOD

本文应用HRP逆行追踪法,结合免疫细胞化学双重标记法,对大鼠延髓尾侧l/3段腹外侧区向伏核的投射进行了研究。将HRP注射到伏核的腹侧部和内侧部,在双侧(以同侧为主)延髓腹外侧区内出现大量的标记细胞,结合免疫细胞化学反应后,在延髓腹外侧区内发现HRP-TH、HRP-NT以及HRP-CCK双标细胞。HRP-TH双标细胞最多,HRP-NT及HRP-ccK双标细胞很少。在孤束核与延髓腹外侧区之间的网状结构内,也发现有少量HRP-TH双标细胞。

By using of combined HRP retrograde tracing and immunocytoche mistry methods, the projection from the caudal part of the ventrolateral medulla (VLM) to the nucleus accumbens (Acb) was examined. When HRP was injected into the ventral and medial area of the caudal part of Acb, the labeled cell bodies were mostly found in the bilateral (predominantly ipsilateral) caudal part of the VLM. When HRP technique (HRP injected into the Acb)was combined with immunocytochemical method, many HRP-TH, HRP-NT and HRP-CCK double labeled cell bodies were found in the VLM. The number of the HRP-TH double labeled cell bodies were numerous, while HRP-NT and HRP-CCK doublelabeled cells were less. HRP-TH double labeled neurons were also found in the reticular formation between the solitary tract nucleus and VLM.