血卟啉衍生物对人胃癌不同周期时相细胞线粒体及琥珀酸脱氢酶的光动力学作用

PHOTODYNAMIC EFFECT OF HEMATOPORPHYRIN DERIVATIVE (HPD) ON MITOCHONDRIA AND SUCCINATE DEHYDROGENASE OF HUMAN GASTRIC CANCER CELLS IN DIFFERENT PHASES OF CELL CYCEL

采用体外培养的人胃癌细胞,用高压N_2O方法及过量TdR的阻断与释放,制备出同步的G_1、s、G_2、M期细胞。各期细胞用血卟啉衍生物(HPD)加红光照射后,在0、24、36、60小时分别用罗丹明123显示活细胞线粒体,同时用细胞化学方法显示细胞内琥珀酸脱氢酶的动态变化。本研究结果表明,同步化各时相细胞在光照后立即观察,线粒体均模糊或消失,而琥珀酸脱氢酶反应并未完全消失,在光敏后24小时最弱,随着时间的推移,先是细胞内线粒体结构逐渐得到恢复,随后才是酶反应的恢复。4个时相细胞中,线粒体和琥珀酸脱氢酶恢复速度依次为s、G_2、G_1、M期,说明HPD对人胃癌细胞的作用有其周期特异性。本文还对HPD光动力学作用于不同周期时相细胞的线粒体损伤、琥珀酸脱氢酶的变化,及对细胞杀伤之间的关系进行了讨论。

Synchronous cells in different phase (G_1, S, G_2, M) of cell cycle were obtained from human gastric low-differentiated mucous adenocarcinoma cells (MGC 80-3), which werec ultured with nitrous oxide under high pressure and blocked with overdosage of TdR. Dynamic changes of the mitochondria stained with Rhodamine-123 and succinate dehydrogenase demonstrated by cytochemical method were observed in various phase of the cells at 0, 24, 36, 60 hours after treatment with HPD plus red light. The results showed that mitochonodria of all four phases are of impairment immediately after photoradiation, SDH reactivity is decreased slightly at 24 hours, the activities of SDH is the weakest. As time goes on, we observed that mitochondria gradually recovered to its original structure and then SDH returned to its normal level. The recovery rate of mitochondria and SDH was in the following order, i. e. S, G_1, G_2, andM. The relationship between these changes and cell killing is briefly discussed.