小鼠胃腺壁细胞超微结构的研究——应用氯离子定位和多糖染色技术

ULTRASTRUCTURAL STUDIES ON PARIETAL CELLS OF MOUSE STOMACH USING CL^- LOCALIZATION AND POLYSACCHARIDES STAINING TECHNIQUES

本文运用氯离子(Cl^-)定位和多糖染色技术,对小鼠胃腺壁细胞超微结构进行了研究。结果发现,Cl^-只定位于壁细胞的内小管和细胞间隙中,小管泡系呈阴性反应;六胺银多糖染色则呈不一致性反应,分别为强阳性、弱阳性或阴性,其阳性反应区只集中于靠近游离面的小管泡系及游离面上。实验结果说明,盐酸分泌过程中还伴随着由小管泡系参与的多糖分泌,而且多糖分泌呈周期性,各壁细胞的这种周期是不同步的。

Cl^- localization and periodic acid-silver methenamine techniques have been used for ultrastructural studies on parietal cells of mouse stomach. Cl^- is located only in the intracellular canaliculi (IC) and intercellular space (IS)of parietal cells. The tubulovesicular system (TS) shows various reactions in periodic acid-silver methenamine staining. The positive reaction was demonstrated in the TS which is located near the apical membranes. This indicates that acid secretion is accompanied by polysaccharides secretion in which the TS takes part, and the polysaccharides secretory activity shows its cyclicity which is characterized by the changes of amounts of secretion. The cycle of parietal cells do not synchronize.