人丘脑及其邻近结构毛细血管的定量研究

QUANTITATIVE STUDY ON THE CAPILLARIES OF THE HUMAN THALAMUS AND ITS ADJACENT STRUCTURES

本研究用4例新生儿脑标本,双色动-静脉连续灌注,硝化棉包埋,制成500μm、100μm和30μm3种厚度交替的连续冠状切片。前者透明封片,后两者Nissl染色,用测微计测量毛细血管内径,图像分析仪测量毛细血管密度,全部数据进行方差分析和相关回归分析。结果表明,丘脑及其邻近结构的毛细血管密度差异十分显著。壳核、外侧膝状体细胞层、丘脑前核和底丘脑核的密度最高;丘脑背内侧核、腹外侧核、枕核、腹后外侧核、后外侧核、中央中核、内侧膝状体、腹前核和内髓板等居中;内囊和外侧膝状体纤维层的最低。在毛细血管直径方面,腹前核和内囊的最粗,壳核和外侧膝状体细胞层的最细。在已测量的同一结构中,毛细血管直径和密度之间存在着负相关。

The brains from four newborns were used in this study. After continuous artery-vein injection with coloured materials, the brains were embedded in nitrocellulose. and coronal sections of 500μm, 100μm and 30μm in thickness were prepared in alternative and successive sequence. The 500μm sections were cleared in wintergreen oil and mounted, and the 100μm and 30μm sections were stained with thionin. The diameter and density of capillaries in thalamus and its adjacent structures were measured by means of Leitz MPV-Tasplus multifunction image analyser. All the data were dealt with variance and correlation analysis statistically. The capillary density of the thalamus and its adjacent structures varied obviously. The value in putamen, cellular layers of lateral geniculate body, anterior thalamic nucleus and subthalamic nucleus was the highest; while the value in dorsomedial nucleus, ventral lateral nucleus, pulvinar nucleus, ventral posterior lateral nucleus, lateral posterior nucleus, centromedian nucleus, medial geniculate body, ventral anterior nucleus and internal medullary lamina was the intermediate; however, the value in internal capsule and fibrous layers of lateral geniculate body was the lowest. The capillary diameter of ventral anterior nucleus and internal capsule was the widest; that of putamen and cellular layers of lateral geniculate body was the smallest; the rest was the intermediate. In the same measured structures, negtive correlation was shown between the capillary diameter and density.